Publications & Reports

MethMarker: user-friendly design and optimization of gene-specific DNA methylation assays.

Peter Schuffler, Thomas Mikeska, Andreas Waha, Thomas Lengauer, Christoph Bock
Max-Planck-Institut fur Informatik, Saarbrucken, Germany. [email protected]

Abstract

DNA methylation is a key mechanism of epigenetic regulation that is frequently altered in diseases such as cancer. To confirm the biological or clinical relevance of such changes, gene-specific DNA methylation changes need to be validated in multiple samples. We have developed the MethMarker http://methmarker.mpi-inf.mpg.de/ software to help design robust and cost-efficient DNA methylation assays for six widely used methods. Furthermore, MethMarker implements a bioinformatic workflow for transforming disease-specific differentially methylated genomic regions into robust clinical biomarkers.

Publication

  • Journal: Genome Biology
  • Published: 05/10/2009
  • Volume: 10
  • Issue: 10
  • Pagination: R105

Author