Publications & Reports

Quality control of astrocyte-directed Cre transgenic mice: the benefits of a direct link between loss of gene expression and reporter activation.

Robert Pascal Requardt, Lech Kaczmarczyk, Pavel Dublin, Anke Wallraff-Beck, Thomas Mikeska, Joachim Degen, Andreas Waha, Christian Steinhauser, Klaus Willecke, Martin Theis
Institute of Genetics, Division of Molecular Genetics, University of Bonn, D-53117 Bonn, Germany.

Abstract

Cre recombinase activity for cell-type restricted deletion of floxed target genes (i.e., flanked by Cre recognition loxP-sites) is often measured by separate matings with recombination-activated reporter gene mice. Using a floxed Gja1 (Cx43) allele, we demonstrate the benefits of a direct link between reporter gene expression and target gene deletion to overcome critical limitations of the Cre/loxP system. The widely used human glial fibrillary acidic protein (hGFAP)-Cre transgene exhibits variable recombination activity and requires postexperimental validation. Such quality control is essential to correlate the extent of Cre-mediated Gja1 ablation with phenotypical alterations and to maintain the activity status of hGFAP-Cre in transgenic mouse colonies. We present several strategies to control for the fidelity of hGFAP-Cre mediated recombination. © 2008 Wiley-Liss, Inc.

Publication

  • Journal: Glia
  • Published: 15/04/2009
  • Volume: 57
  • Issue: 6
  • Pagination: 680-692

Author