Publications & Reports

Distinct cytokine patterns in semen influence local HIV shedding and HIV target cell activation.

Abraham J Olivier, Lindi Masson, Katharina Ronacher, Gerhard Walzl, David Coetzee, David A Lewis, Anna-Lise Williamson, Jo-Ann S Passmore, Wendy A Burgers
Institute of Infectious Disease and Molecular Medicine and Division of Medical Virology, University of Cape Town.

Abstract

BACKGROUND: Semen is the main vector for human immunodeficiency virus (HIV) transmission from men to women. We investigated the influence of cytokines in semen on local HIV burden and activated T cells. METHODS: Blood and semen were collected from 42 HIV-negative and 38 HIV-positive men. Concentrations of 20 cytokines were measured by Luminex, and frequencies of activated T cells were measured by flow cytometry. RESULTS: Semen contained higher concentrations of proinflammatory (monocyte chemotactic protein-1, interleukin [IL]-8, IL-6, Fractalkine, macrophage inflammatory protein (MIP)-1beta, granulocyte macrophage colony-stimulating factor) and adaptive cytokines (IL-7 and IL-15) and higher frequencies of activated T cells compared to blood. Plasma IL-2, eotaxin, MIP-1beta, and IL-15 and semen eotaxin and granulocyte colony-stimulating factor (G-CSF) concentrations were associated with T-cell activation. Cytokines in semen were highly coregulated in HIV-negative men; however, this network was disrupted during HIV infection. Several cytokines in semen correlated with HIV shedding (G-CSF, tumor necrosis factor-alpha [TNF-alpha], interferon-gamma [IFN-gamma], IL-10). CONCLUSION: Higher levels of inflammation and T-cell activation were observed in semen compared with blood. Seminal G-CSF, which influences neutrophil survival, T-cell function, and dendritic cell activation, was associated with T-cell activation and HIV shedding and may be an important target for reducing HIV shedding or risk.

Publication

  • Journal: The Journal of Infectious Diseases
  • Published: 15/04/2014
  • Volume: 209
  • Issue: 8
  • Pagination: 1174-1184

Author