Publications & Reports

APELIN promotes hematopoiesis from human embryonic stem cells.

Yu QC, Hirst CE, Costa M, Ng ES, Schiesser JV, Gertow K, Stanley EG, Elefanty AG

Abstract

Transcriptional profiling of differentiating human embryonic stem cells (hESCs) revealed that MIXL1-positive mesodermal precursors were enriched for transcripts encoding the G-protein-coupled APELIN receptor (APLNR). APLNR-positive cells, identified by binding of the fluoresceinated peptide ligand, APELIN (APLN), or an anti-APLNR mAb, were found in both posterior mesoderm and anterior mesendoderm populations and were enriched in hemangioblast colony-forming cells (Bl-CFC). The addition of APLN peptide to the media enhanced the growth of embryoid bodies (EBs), increased the expression of hematoendothelial genes in differentiating hESCs, and increased the frequency of Bl-CFCs by up to 10-fold. Furthermore, APLN peptide also synergized with VEGF to promote the growth of hESC-derived endothelial cells. These studies identified APLN as a novel growth factor for hESC-derived hematopoietic and endothelial cells.

Link to publisher’s web site

Publication

  • Journal: Blood
  • Published: 28/06/2012
  • Volume: 119
  • Issue: 26
  • Pagination: 6243-6254

Author