Publications & Reports

The impact of Nucleofection(R) on the activation state of primary human CD4 T cells.

Zhang M, Ma Z, Selliah N, Weiss G, Genin A, Finkel TH, Cron RQ
Division of Pediatric Rheumatology, University of Alabama at Birmingham, 1825, University Blvd., Shelby Building, Rm. 371, Birmingham, AL 35233, United States. Electronic address: mzhang@peds.uab.edu.

Abstract

Gene transfer into primary human CD4 T lymphocytes is a critical tool in studying the mechanism of T cell-dependent immune responses and human immunodeficiency virus-1 (HIV-1) infection. Nucleofection® is an electroporation technique that allows efficient gene transfer into primary human CD4 T cells that are notoriously resistant to traditional electroporation. Despite its popularity in immunological research, careful characterization of its impact on the physiology of CD4 T cells has not been documented. Herein, using freshly-isolated primary human CD4 T cells, we examine the effects of Nucleofection® on CD4 T cell morphology, intracellular calcium levels, cell surface activation markers, and transcriptional activity. We find that immediately after Nucleofection®, CD4 T cells undergo dramatic morphological changes characterized by wrinkled and dilated plasma membranes before recovering 1hour later. The intracellular calcium level also increases after Nucleofection®, peaking after 1hour before recovering 8hours post transfection. Moreover, Nucleofection® leads to increased expression of T cell activation markers, CD154 and CD69, for more than 24hours, and enhances the activation effects of phytohemagglutinin (PHA) stimulation. In addition, transcriptional activity is increased in the first 24hours after Nucleofection®, even in the absence of exogenous stimuli. Therefore, Nucleofection® significantly alters the activation state of primary human CD4 T cells. The effect of transferred gene products on CD4 T cell function by Nucleofection® should be assessed after sufficient resting time post transfection or analyzed in light of the activation caveats mentioned above.

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Publisher’s pdf available at http://www.sciencedirect.com.ezp.lib.unimelb.edu.au/science/article/pii/S0022175914001768

Publication

  • Journal: Journal of Immunological Methods
  • Published: 05/06/2014
  • Volume: 408
  • Pagination: 123-31

Author

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