Publications & Reports

Cold adaptation improves the growth of seasonal influenza B vaccine viruses.

Kim H, Schoofs P, Anderson DA, Tannock GA, Rockman SP
Centre for Biomedical Research, Burnet Institute, 85 Commercial Road, Melbourne 3004, VIC, Australia; School of Applied Sciences, RMIT University, Plenty Road, Bundoora 3083, VIC, Australia. Electronic address: [email protected]


Gene reassortment has proved useful in improving yields of influenza A antigens of egg-based inactivated vaccines, but similar approaches have been difficult with influenza B antigens. Current regulations for influenza vaccine seed viruses limit the number of egg passages and as a result resultant yields from influenza B vaccine seed viruses are frequently inconsistent. Therefore, reliable approaches to enhance yields of influenza B vaccine seed viruses are required for efficient vaccine manufacture. In the present study three stable cold-adapted (ca) mutants, caF, caM and caB derived from seasonal epidemic strains, B/Florida/4/2006, B/Malaysia/2506/2004 and B/Brisbane/60/2008 were prepared, which produced high hemagglutinin antigen yields and also increased viral yields of reassortants possessing the desired 6:2 gene constellation. The results demonstrate that consistent improvements in yields of influenza B viruses can be obtained by cold adaptation following extended passage. Taken together, the three ca viruses were shown to have potential as donor viruses for the preparation of high-yielding influenza B vaccine viruses by reassortment.


  • Journal: Vaccine
  • Published: 11/03/2014
  • Volume: 32
  • Issue: 21
  • Pagination: 2474-2479