Publications & Reports

Sindbis virus vectors elicit hemagglutinin-specific humoral and cellular immune responses and offer a dose-sparing strategy for vaccination.

Annett Miller, Rob J Center, John Stambas, Georgia Deliyannis, Peter C Doherty, Jane L Howard, Stephen J Turner, Damian F J Purcell
Department of Microbiology and Immunology, University of Melbourne, Parkville 3010, Australia.

Abstract

We report here on the use of a Sindbis virus-based DNA-launch RNA replicon vector (pSIN-HA) that expresses influenza hemagglutinin (HA) as an immunogen. Immunization of mice with pSIN-HA generated anti-HA antibody and CTL responses and resulted in lower lung viral titers after influenza challenge when compared to controls. Importantly, immunization with a low dose of pSIN-HA mediated significantly reduced lung viral titers following challenge at 43 weeks after the final immunization. In contrast, immunization with a non-replicon DNA vector expressing HA failed to mediate reduced lung viral titer at the same dose. This demonstrated the dose-sparing capacity of the SIN vector system and its ability to stimulate long-term memory responses, properties that are highly desirable in any vaccine formulation.

Publication

  • Journal: Vaccine
  • Published: 16/10/2008
  • Volume: 26
  • Issue: 44
  • Pagination: 5641-5648

Author