Publications & Reports

Induction of HIV-1 subtype B and AE-specific neutralizing antibodies in mice and macaques with DNA prime and recombinant gp140 protein boost regimens.

Rob J Center, Adam K Wheatley, Shahan M Campbell, Adriana J Gaeguta, Viv Peut, Sheilajen Alcantara, Carly Siebentritt, Stephen J Kent, Damian F J Purcell
Department of Microbiology and Immunology, University of Melbourne, Parkville, 3010 VIC, Australia.

Abstract

We developed highly expressing clade B and AE DNA and envelope protein (Env) vaccines for evaluation in mice and macaques as DNA prime/protein boost regimens. High levels of Env-specific antibodies were induced in mice, albeit with limited neutralizing activity in vitro. A combined clade B and AE regimen induced high titer Env-specific antibody in two pigtail macaques that neutralized several strains of HIV-1. However, upon mucosal challenge with SHIV(SF162P3) no protection from infection was observed. Although the vaccines tested provide a platform for inducing robust humoral immunity, further refinements to broaden coverage against divergent strains and induce mucosal immunity are needed.

Publication

  • Journal: Vaccine
  • Published: 05/11/2009
  • Volume: 27
  • Issue: 47
  • Pagination: 6605-6612

Author

Health Issue