Publications & Reports

Induction of humoral and cellular immune responses against the HIV-1 envelope protein using gamma-retroviral virus-like particles.

Tea Kirkegaard, Adam Wheatley, Jesper Melchjorsen, Shervin Bahrami, Finn S Pedersen, Robert J Center, Damian F J Purcell, Lars Ostergaard, Mogens Duch, Martin Tolstrup
Department of Infectious Diseases, Aarhus University Hospital, Skejby, DK-8200 Aarhus N, Denmark.

Abstract

This study evaluates the immunogenicity of the HIV envelope protein (env) in mice presented either attached to gamma-retroviral virus-like-particles (VLPs), associated with cell-derived microsomes or as solubilized recombinant protein (gp160). The magnitude and polyfunctionality of the cellular immune response was enhanced when delivering HIV env in the VLP or microsome form compared to recombinant gp160. Humoral responses measured by antibody titres were comparable across the groups and low levels of antibody neutralization were observed. Lastly, we identified stronger IgG2a class switching in the two particle-delivered antigen vaccinations modalities compared to recombinant gp160.

Publication

  • Journal: Virology Journal
  • Published: 01/08/2011
  • Volume: 8
  • Pagination: 381

Author

Health Issue