Publications & Reports

Increased glucose metabolic activity is associated with CD4+ T-cell activation and depletion during chronic HIV infection.

Palmer CS, Ostrowski M, Gouillou M, Tsai L, Yu D, Zhou J, Henstridge DC, Maisa A, Hearps AC, Lewin SR, Landay A, Jaworowski A, McCune JM, Crowe SM
aCentre for Biomedical Research, Burnet Institute, Melbourne, Australia bInstituto de Investigaciones Biomedicas en Retrovirus y SIDA. Facultad de Medicina, Buenos Aires, Argentina cCentre for Population Health, Burnet Institute, Melbourne dDepartment of

Abstract

OBJECTIVES:: Glucose metabolism plays a fundamental role in supporting the growth, proliferation and effector functions of T cells. We investigated the impact of HIV infection on key processes that regulate glucose uptake and metabolism in primary CD4+ and CD8+ T cells. DESIGN AND METHODS:: Thirty-eight HIV-infected treatment-naive, 35 HIV+/combination antiretroviral therapy, 7 HIV+ long-term nonprogressors and 25 HIV-control individuals were studied. Basal markers of glycolysis [e.g. glucose transporter-1 (Glut1) expression, glucose uptake, intracellular glucose-6-phosphate, and L-lactate] were measured in T cells. The cellular markers of immune activation, CD38 and HLA-DR, were measured by flow cytometry. RESULTS:: The surface expression of the Glut1 is up-regulated in CD4+ T cells in HIV-infected patients compared with uninfected controls. The percentage of circulating CD4+Glut1+ T cells was significantly increased in HIV-infected patients and was not restored to normal levels following combination antiretroviral therapy. Basal markers of glycolysis were significantly higher in CD4+Glut1+ T cells compared to CD4+Glut1- T cells. The proportion of CD4+Glut1+ T cells correlated positively with the expression of the cellular activation marker, HLA-DR, on total CD4+ T cells, but inversely with the absolute CD4+ T-cell count irrespective of HIV treatment status. CONCLUSION:: Our data suggest that Glut1 is a potentially novel and functional marker of CD4+ T-cell activation during HIV infection. In addition, Glut1 expression on CD4+ T cells may be exploited as a prognostic marker for CD4+ T-cell loss during HIV disease progression.

The authors gratefully acknowledge the contribution to this work of the Victorian Operational Infrastructure Support Program received by the Burnet Institute.Sources of support: This research was funded by a 2010 developmental grant (CNIHR) from the University of Washington Center for AIDS Research (CFAR), an NIH funded program under award number AI027757 which is supported by the following NIH Institutes and Centers (NIAID, NCI, NIMH, NIDA, NICHD, NHLBI, NIA), and the Australian Centre for HIV and Hepatitis Virology Research (ACH2). C.S.P is a recipient of the CNIHR and ACH2 grant. SMC is a recipient of a National Health and Medical Research Council of Australia (NHMRC) Principal Research Fellowship.

Full text of this article can be obtained from the publisher’s web site at:

http://journals.lww.com/aidsonline/pages/articleviewer.aspx?year=2014&issue=01280&article=00002&type=abstract

Publication

  • Journal: AIDS
  • Published: 01/01/2014
  • Volume: 28
  • Issue: 3
  • Pagination: 297-309

Author

Health Issue