Publications & Reports

Evolution of B Cell Responses to Clec9A-Targeted Antigen.

Park HY, Light A, Lahoud MH, Caminschi I, Tarlinton DM, Shortman K
The Walter and Eliza Hall Institute, Parkville, Victoria 3052, Australia;


The response of B cells to Ag targeted to Clec9A on dendritic cells was followed using the hapten nitrophenol (NP) conjugated to rat Ig carrier. Injection of small amounts of NP conjugated to anti-Clec9A in the absence of adjuvants gave high and very prolonged Ab responses, approaching those obtained by high doses of nontargeted NP-protein conjugates with alum adjuvant. The response to NP-anti-Clec9A included the transient formation of germinal centers, maturation of Ab affinity, and some memory B cell formation. Serum Ab titers remained high 35 wk postimmunization, well after the initial follicular response had faded. The results suggest Clec9A-targeting strategies for improving Ab responses to vaccine Ags.

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  • Journal: Journal of Immunology
  • Published: 11/10/2013
  • Volume: 191
  • Issue: 10
  • Pagination: 4919-4925