Publications & Reports

Identification of two novel human immunodeficiency virus type 1 splice acceptor sites in infected T cell lines.

Smith J, Azad A, Deacon N
AIDS Molecular Biology Laboratory, Macfarlane Burnet Centre for Medical Research, Fairfield Hospital, Victoria, Australia.

Abstract

The regulatory and accessory proteins of human immunodeficiency virus type 1 (HIV-1) are produced from singly or multiply spliced mRNAs. We have used HIV-1-specific oligonucleotide primer pairs in a polymerase chain reaction procedure on RNA from lymphocyte cell lines infected with HIV-1(228,200). The amplified products were analysed by hybridization with splice junction-specific oligonucleotide probes to determine splice donor/splice acceptor combination utilization, subcloned into a plasmid vector and the nucleotide sequences were obtained. Two novel splice acceptor sites have been identified.

Publication

  • Journal: The Journal of General Virology
  • Published: 01/07/1992
  • Volume: 73 ( Pt 7)
  • Pagination: 1825-1828