Publications & Reports

T cell lysis of murine renal cancer: multiple signaling pathways for cell death via Fas.

Sayers TJ, Brooks AD, Seki N, Smyth MJ, Yagita H, Blazar BR, Malyguine AM
Intramural Research Support Program, SAIC-Frederick, DBS, NCI-FCRDC, Maryland 21702-1201, USA. Sayers@mail.ncifcrf.gov

Abstract

Activated T cells lyse the murine renal cancer Renca. We have examined the mechanism of tumor cell lysis with the use of T cells derived from C57BL/6, BALB/c, B6.gld, and B6.Pfp-/- mice. C57BL/6 and BALB/c T cells can lyse Renca cells through the use of both granule- and Fas ligand (FasL)-mediated pathways. However, B6.gld T cells predominantly use granule-mediated killing, whereas B6.Pfp-/- T cells use FasL. The lysis of Renca by Pfp-/- T cells is only partially inhibited by the caspase inhibitor ZVAD-FMK, suggesting that caspase-independent signaling is also important for Renca cell lysis. When the reactive oxygen scavenger butylated hydroxyanisole was used alone or in combination with ZVAD-FMK a substantial reduction of Renca lysis was observed. Therefore, the caspase-independent generation of reactive oxygen intermediates in Renca after Fas triggering contributes to the lysis of these cells.

Publication

  • Journal: Journal of Leukocyte Biology
  • Published: 01/07/2000
  • Volume: 68
  • Issue: 1
  • Pagination: 81-86