Publications & Reports

Visualisation of phenotypically mixed HIV-1 and HTLV-I virus particles by electron microscopy.

Lawson VA, Lee JY, Doultree JC, Marshall JA, McPhee DA
AIDS Cellular Biology Unit, National Centre for HIV Virology Research, Macfarlane Burnet Centre for Medical Research, Fairfield, Australia. lawson@burnet.edu.au

Abstract

Virions produced after HIV-1 infection of HTLV-I transformed cells have an expanded tropism that has been attributed to the presence of HTLV-I glycoproteins in the envelope. This report now directly identifies these phenotypically mixed virions by immunogold labelling electron microscopy. Furthermore we estimate there are 2% of these in cell-free supernatant, which represents up to 1 x 10(7) particles/ml from an in vitro infection. HTLV-1 envelope labelling was localised to a single region, suggesting a defined event in packaging of foreign envelope proteins into HIV-1 virus particles.

Publication

  • Journal: Journal of Biomedical Science
  • Published: 01/01/2000
  • Volume: 7
  • Issue: 1
  • Pagination: 71-74

Health Issue