Publications & Reports

Vaccination with attenuated simian immunodeficiency virus by DNA inoculation.

Kent SJ, Dale CJ, Preiss S, Mills J, Campagna D, Purcell DF
Department of Microbiology and Immunology, University of Melbourne, Parkville, Victoria 3010, Australia. skent@unimelb.edu.au

Abstract

Delivering attenuated lentivirus vaccines as proviral DNA would be simple and inexpensive. Inoculation of macaques with wild-type simian immunodeficiency virus strain mac239 (SIV(mac239)) DNA or SIV(mac239) DNA containing a single deletion in the 3' nef-long terminal repeat overlap region (nef/LTR) led to sustained SIV infections and AIDS. Injection of SIV(mac239) DNA containing identical deletions in both the 5' LTR and 3' nef/LTR resulted in attenuated SIV infections and substantial protection against subsequent mucosal SIV(mac251) challenge.

Publication

  • Journal: Journal of Virology
  • Published: 01/12/2001
  • Volume: 75
  • Issue: 23
  • Pagination: 11930-11934