Publications & Reports

Culture of HIV in monocytes and macrophages.

Crowe S, Maslin C, Ellery P, Kedzierska K
Macfarlane Burnet Institute for Medical Research and Monash University, Melbourne, Australia.


Monocytes and macrophages constitute important cellular targets for macrophage-tropic (M-tropic) strains of HIV-1 and are believed to play a major role in the pathogenesis of AIDS. This updated unit provides procedures for culturing HIV in these target cells under suspension and adherent cell culture conditions. The unit also provides methods for expanding M-tropic primary isolates of HIV and determining the 50% tissue culture infectious dose (TCID50) of HIV stocks.


  • Journal: Current protocols in Immunology
  • Published: 01/09/2004
  • Volume: Chapter 12
  • Pagination: Unit 12.4

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