Publications & Reports

In silico directed mutagenesis identifies the CD81/claudin-1 hepatitis C virus receptor interface.

Davis C, Harris HJ, Hu K, Drummer HE, McKeating JA, Mullins JG, Balfe P

Abstract

Hepatitis C virus (HCV) entry is dependent on host cell molecules tetraspanin CD81, scavenger receptor BI and tight junction proteins claudin-1 and occludin. We previously reported a role for CD81/claudin-1 receptor complexes in HCV entry, however, the molecular mechanism(s) driving association between the receptors is unknown. We explored the molecular interface between CD81 and claudin-1 using a combination of bioinformatic sequence based modelling, site-directed mutagenesis, and Fluorescent Resonance Energy Transfer (FRET) imaging methodologies. Structural modelling predicts the first extracellular loop of claudin-1 to have a flexible beta conformation and identifies a motif between amino acids 62-66 that interacts with CD81 residues T149, E152 and T153. FRET studies confirm a role for these CD81 residues in claudin-1 association and HCV infection. Importantly, mutation of these CD81 residues has minimal impact on protein conformation or HCV glycoprotein binding, highlighting a new functional domain of CD81 that is essential for virus entry.

Publication

  • Journal: Cellular Microbiology
  • Published: 17/08/2012
  • Volume: 14
  • Issue: 12
  • Pagination: 1892-1903

Author