Projects

HIV reverse transcriptase structure function and role in HIV replication

We are studying the impact of mutations and small molecule inhibitors that modulate RT subunit interaction on enzyme function and HIV-1 replication.

Our studies on the molecular determinants of RT dimerization have identified a region in the RT that is critical for RT dimerization, enzyme function and virion RT maturation.

Using the yeast two-hybrid system we have found that nonnucleoside reverse transcriptase inhibitors (NNRTIs) can enhance RT dimerization, which may contribute in part to the inhibitory effect of these drugs.

We are also studying the role of the reverse transcriptase embedded in Gag-Pol on activation of the HIV protease and Gag-Pol processing leading to the production of infectious HIV particles and the role of host cell factors in this process.

Publications

2008

2006

2005

2003

2001

2000

Collaborators

  • Nicolas Sluis-Cremer, University of Pittsburgh, PA, USA
  • Michael Kuiper, VPAC

Funding

NHMRC Project Grant: 235030 Molecular Interactions Between the Subunits of the HIV Reverse Transcriptase

NHMRC Project Grant: 381705 HIV Maturation

Health Issue

Contact Details

For any general enquiries relating to this project, please contact:

Professor Gilda Tachedjian

Head of Life Sciences; Head of Tachedjian Laboratory (Retroviral Biology and Antivirals)

Telephone

+61392822256

Email

[email protected]